Credit: Horticulture Research
The breeding process of MAS for backcrossing to introduce translocation chromosome. (A) HMF was the backcross parent variety. The red text presented the heterozygous translocation line (Aa) and homozygous translocation line (AA) by MAS in each generation. (B) Heterozygous translocation lines (Aa) were screened on the BC1 population using the translocation recombination marker Chr10 + Chr106; Homozygous translocation lines (AA) were screened on the BC4F2 population. (C) Wild-type HMM and homozygous translocation line HMF-TL were used to prepare the hybrid combination “Less-seed HM F1”. Compared with “CK HM F1”, which used wild-type “HMM” and wild-type “HMF” as the paternal parent to prepare the hybrid group, the fruit transection section seed number ratio of “Less-seed HM F1” was significantly reduced.