Credit: Light: Advanced Manufacturing
Simplified overview of the optical setup. The rays of the fluorescence particle are refracted by the fluctuating water-air interface. Because this aberrating layer can be considered planar, the correction performance can be increased by placing the deformable mirror in a conjugate plane to the water-air interface. The height profile of the water surface is sampled with a probe beam (green), which we term Fresnel Guide Star (FGS), and a wavefront sensor. The probe beam (FGS) is coupled into the system via a beam splitter and its reflex at the water-air interface is imaged to the deformable mirror and the wavefront sensor. For the sake of simplicity, the 3D localisation microscope is illustrated here as 2D microscope.