With a high sensitivity and precision, this approach uses a 96-well microplate format for a batch of tests which are capable of being run in a manual, semi-automated, or fully automated capacity. This high-throughput stable assay is user-friendly, cost-effective and has excellent correlation to the existing commercial methods.
As a precursor of fat-soluble steroid hormone, Vitamin D is involved in the intestinal absorption of calcium and the regulation of calcium homeostasis in the blood. Vitamin D in the human body is mainly from food or produced when the skin is exposed to sunlight. 25-OH Vitamin D is the most ideal indicator to show the nutrition of Vitamin D in the human blood. It is the main circulation form of Vitamin D, and is produced by Vitamin D catalyzed by Vitamin D-25-hydroxylase in the liver. Therefore, testing the level of 25-OH Vitamin D in the serum is used to evaluate and to help people know the nutrition of Vitamin D in the body, and to diagnose and to monitor the treatment effects on osteoporosis, rickets, osteomalacia, renal bone dystrophy and other diseases.