ADSC-Exos outperform BMSC-Exos in alleviating hydrostatic pressure-induced injury to retinal ganglion cells by upregulating nerve growth factors

BACKGROUND

Mesenchymal stem cells (MSCs) have protective effects on the cornea, lacrimal gland, retina, and photoreceptor cell damage, which may be mediated by exosomes (exos) released by MSCs.

AIM

To investigate the ameliorating effect of exos derived from different MSCs on retinal ganglion cell (RGC) injury induced by hydrostatic pressure.

METHODS

The RGC injury model was constructed by RGC damage under different hydrostatic pressures (40, 80, 120 mmHg). Then RGCs were cultured with adipose-derived stem cell (ADSC)-Exos and bone marrow-derived stem cell (BMSC)-Exos. Cell Counting Kit-8, transmission electron microscopy, flow cytometry, immunofluorescence, real-time quantitative polymerase chain reaction, and western blotting were performed to detect the ameliorating effect of exos on pressure-induced RGC injury.

RESULTS

ADSC-Exos and BMSC-Exos were successfully isolated and obtained. The gibbosity of RGCs was lower, the cells were irregularly ellipsoidal under pressure, and the addition of ADSC-Exos and BMSC-Exos significantly restored RGC morphology. Furthermore, the proliferative activity of RGCs was increased and the apoptosis of RGCs was inhibited. Moreover, the levels of lactate dehydrogenase and apoptosis-related proteins were increased, and the concentrations of antiapoptotic proteins and neurotrophic factors were decreased in damaged RGCs. However, the above indicators were significantly improved after ADSC-Exos and BMSC-Exos treatment.

CONCLUSION

These findings indicated that ADSC-Exos and BMSC-Exos could ameliorate RGC injury caused by hydrostatic pressure by inhibiting apoptosis and increasing the secretion of neurotrophic factors.

Key Words: Adipose-derived stem cells, Bone marrow-derived stem cells, Exosomes, Glaucoma

Core Tip: We discovered for the first time that adipose-derived stem cell-exosomes (ADSC-Exos) significantly ameliorate retinal ganglion cell (RGC) injury caused by hydrostatic pressure by inhibiting apoptosis and increasing the secretion of neurotrophic factors. ADSC-Exos manifested better ameliorating effects than bone marrow-derived stem cell (BMSC)-Exos in ameliorating the RGC injury induced by hydrostatic pressure. BMSC-Exos ameliorate optic nerve injury caused by hydrostatic pressure by inhibiting apoptosis and increasing the secretion of neurotrophic factors.

• Citation: Zheng ZK, Kong L, Dai M, Chen YD, Chen YH. ADSC-Exos outperform BMSC-Exos in alleviating hydrostatic pressure-induced injury to retinal ganglion cells by upregulating nerve growth factors. World J Stem Cells 2023; 15(12): 1077-1092
• URL: https://www.wjgnet.com/1948-0210/full/v15/i12/1077.htm
• DOI: https://dx.doi.org/10.4252/wjsc.v15.i12.1077