The discovery contributes to the understanding of cell membrane properties, and in the long term, could inform treatment of neurodegenerative diseases, said Roger Koeppe II, Distinguished Professor in the Department of Chemistry and Biochemistry at the U of A.
The study was published in the journal Biochemistry.
Jordana Thibado, a recent honors graduate of the U of A, was the study’s lead author. Her discovery builds on work conducted by researchers in Koeppe’s lab, who found that a similar amino acid, lysine, has a tendency to release its positive charge when inserted into lipids that represent the cell membrane environment.
“Forming a model to understand the physics of transmembrane proteins is extremely important for understanding physiology that we rely on every millisecond,” Thibado said. “Down the line, this work could advance drug design for diseases like Alzheimer’s and Parkinson’s where protein aggregation can occur.”
Thibado inserted arginine into models of two peptide helices that mimic protein components of the cell membrane, at different positions on the helix. Thibado then explored what happened when the helices were exposed to cholesterol. “The future challenge is to figure out why cholesterol has such a big effect on one of these samples, and not on the other, because we don’t understand why this is happening,” Koeppe said. “Cholesterol is much studied, but many of the fundamental properties still are not well understood.”
In addition to Koeppe, the co-authors included U of A doctoral student Ashley Martfeld and research associate professor Denise Greathouse.
Thibado is now pursuing a doctoral degree in physiology, biophysics and systems biology at Weill Medical College, Cornell University’s medical campus in New York City.
About the University of Arkansas: The University of Arkansas provides an internationally competitive education for undergraduate and graduate students in more than 200 academic programs. The university contributes new knowledge, economic development, basic and applied research, and creative activity while also providing service to academic and professional disciplines. The Carnegie Foundation classifies the University of Arkansas among only 2 percent of universities in America that have the highest level of research activity. U.S. News & World Report ranks the University of Arkansas among its top American public research universities. Founded in 1871, the University of Arkansas comprises 10 colleges and schools and maintains a low student-to-faculty ratio that promotes personal attention and close mentoring.
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