“This novel technique allows for far more efficient gene editing of stem cells and will increase the speed of new discoveries in the field,” said co-senior author Clive Svendsen, PhD, director of the Board of Governors Regenerative Medicine Institute.
The irradiation method could prove effective in learning more about diseases such as spinal muscular atrophy, muscular dystrophy and Huntington’s disease. Gene editing allows scientists to correct disease causing mutations and, theoretically, cure the disease in the petri dish. Additionally, gene-editing technology allows scientists to create disease mutations in normal cells, thus modeling human disease.
When using this form of gene editing, Cedars-Sinai scientists can more efficiently insert reporter genes that glow when a stem cell turns into a specific cell of the body. For example, stem cells would turn green when converted into a heart cell and red when turned into a neuron.
“The combination of low-dose irradiation and correct gene copy will accelerate our ability to model human disease using stem cells from patients with many different disorders,” said co-senior author Vaithilingaraja Arumugaswami, MVSc, PhD, director of the Pancreas and Liver Program in the Cedars-Sinai Board of Governors Regenerative Medicine Institute.
Over the past few years, the field of creating human diseases in the dish using stem cells has expanded rapidly. This work allows scientists to test novel drugs on human cells that carry disease-causing genes.
“Radiation, which is normally considered harmful, has proven beneficial in gene editing,” said Svendsen. This new technique will help us establish far more accurate models and accelerate the discovery process.”.
Additional Cedars-Sinai scientists involved in the study include lead project scientist Seigo Hatada, PhD; Aparna Subramanian, PhD; Berhan Mandefro; Songyang Ren, MD, PhD; Ho Won Kim, PhD; Jay (Jie) Tang, PhD; Vincent Funari, PhD; Robert Baloh, MD, PhD; and Dhruv Sareen, PhD.
Funding for this novel work was supported by a Cedars-Sinai programmatic award and the ALS Association.
Citation: Stem Cells Translational Medicine: 2015 July: Low dose irradiation enhances gene targeting in human pluripotent stem cells.
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